Why do we use ROCK inhibitors in stem cell culture?
Biology · High School · Mon Jan 18 2021
Answered on
Explanation: Stem cell culture condition must allow the establishment and the maintenance of phenotypically well defined and karyotipycally stable cells. The condition must maintain self-renewal and pluri/multipotency potentials. Cell cultures conditions need to be standardized and use well defined matrices and media.
The mechanism of ROCK inhibitors' ability to decrease IOP is through direct action on the trabecular meshwork and Schlemm's canal cells (increases permeability), which play a large role in the resistance of aqueous humor outflow.
ROCK inhibition enables maintenance of stem cell phenotype; its effects on metabolism are unknown.