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Either in E. coli or Saccharomyces cerevisiae kind of organism is used go produce human insulin .
Explanation: Using E. coli expression system, the insulin precursors (IP) are produced as inclusion bodies and fully functional polypeptides are obtained finally by solubilization and refolding procedures.
Saccharomyces cerevisiae is the most preferred and predominant yeast for large scale commercial production of insulin, however several other alternate yeast strains have been explored for insulin productions .