This is an artificial genetic sequence from combining two other sequences in a plasmid.
Biology · High School · Thu Feb 04 2021
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Correct answer is recombination DNA .
- Steps of recombinant DNA technology;
- Isolation of genetic material i.e; isolation of the DNA fragments or genes to be cloned
- Restriction enzyme digestion
- Amplification
- Ligation
- Insertion of recombinant DNA into host i.e; insertion of the isolated gene in a suitable plasmid vector.
- Transformation i.e; Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation).
- Obtaining foreign gene product i.e; multiplication/expression of the introduced gene in the host.
- Down streaming process i.e; Selection of the transformed host cells and identification of the clone containing the desired gene/DNA fragment.